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Biomolecular recognition, the process by which biomolecules recognize and bind to their molecular targets, typically highly specific, high affinity and reversible, and is generalizable to an effectively unlimited range of aqueous analytes. Consequently, it has been exploited in a wide range of diagnostic and synthetic technologies. Biomolecular recognition is typically driven by many weak interactions...
The quality of a biosensing system relies on the interfacial properties where bioactive species are immobilized. The design of the surface includes both the immobilization of the bioreceptor itself and the overall chemical preparation of the transducer surface. Hence, the sensitivity and specificity of such devices are directly related to the accessibility and activity of the immobilized molecules...
Development and improvement of surface-sensitive biosensors is made possible by the use of analytical tools. Spectroscopies available today are known for their extreme sensitivity and quantitation of functional chemical groups within the first nanometers of the surface. The first part of this chapter highlights Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) based on the detection of ionic...
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